Publications - Clémence Bourély

Toxoplamosis

Development and evaluation of a modified agglutination test for diagnosis of Toxoplasma infection using tachyzoites cultivated in cell culture. Batol H. Al-Adhami | Manon Simard | Adrián Hernández-Ortiz | Clémence Boireau | Alvin A. Gajadhar. Food and Waterborne Parasitology, Volume 2, March 2016, Pages 15–21.

Highlights:

• An in-house MAT was developed using tachyzoites produced by in vitro cultivation.

• The assay was used to test samples of serum, blood and/or meat juice from variety of hosts.

• The performance of this assay was shown to be similar to that of a commercial MAT.

• The in-house MAT is a simple, rapid, inexpensive and multi-host species diagnostic tool.

Tuberculosis

Assessment of the intrinsic characteristic of the gamma-interferon test used in serial after a first skin test for the diagnosis of bovin tuberculosis in France and sociological survey of the local stakeholders. Clémence Boireau. Doctorate in Veterinary medicine, 2015.

Sensitivity of γ-interferon test used in series after tuberculin test to detect bovine tuberculosis. Anne Praud | Clémence Boireau | Barbara Dufour. Veterinary Record, August 2016 ; 179(7):174. doi: 10.1136/vr.103803.

Highlights:

• The gamma-interferon test (IFND3) performed between three and eight days after a non-negative result to

screening STs was significantly more sensitive than the usual test (single comparative cervical tuberculin test, 42 days later).

• The serial association of screening skin test with IFND3 could thus be used to shorten the lock-up of suspect herds without subdetecting infected cattle.

Analyse qualitative de la participation volontaire des éleveurs français au protocole expérimental d’évaluation du test interféron-gamma. Clémence Boireau | Anne Praud. Épidémiol. et santé anim., 2016, 69, 105-122

Pourquoi les éleveurs français ont-ils participé au protocole expérimental d’évaluation du test interféron gamma ? Analyse des facteurs de succès et d’échec. Clémence Boireau | Anne Praud. AEEMA meeting, March 2016. Oral communication.

The importance of communication in promoting voluntary participation in an experimental trial. Clémence Boireau | Barbara Dufour | Anne Praud. Plos One, 2017, 12, e0185799.

Antimicrobial resistance

Highlights:

• By applying time-series analysis, we described antimicrobial resistance trends of pathogenic Escherichia coli in food-producing animals: the data generated can help in designing efficient control strategies and policies.

• The results highlighted the need to set up national surveillance networks able to monitor antibiotic resistance of pathogens in food-producing animals

• Resistance to third-generation cephalosporins emerged during the period of the study: this trend is particularly alarming because these antimicrobial agents constitute last resort antibiotics for some human diseases.

Le Résapath est-il représentatif ? Clémence Boireau. RESAPATH meeting, 5 December 2017. Oral communication.

Quels sont les déterminants du recours à l’antibiogramme en santé animale en France ? Clémence Boireau. RESAPATH meeting, 5 December 2017. Oral communication.

Highlights:

• This study aim to estimate the AMR levels of the three main bacteria isolated from dairy cattle with mastitis in France: Streptococcus uberis, Escherichia coli, and coagulase-positive staphylococci
• In E. coli, the highest proportions of resistance were observed for amoxicillin (28.1%) and tetracycline (23.1%).
• Resistance to third-generation cephalosporins in E. coli from dairy cattle was almost nil in 2006, but reached 2.4% in December 2016.

Highlights:

• RESAPATH is a powerful tool for monitoring animal-host antimicrobial resistance.
• A time-series analysis depicted various trends of E. coli resistance to 3GCs.
• After a peak of 26% in 2010, resistance in broilers rapidly decreased to below 5%.
• Our findings demonstrate that inversion of resistance trends can rapidly occur.

Why do veterinarians ask for antimicrobial susceptibility testing? A qualitative study exploring determinants and evaluating the impact of antibiotic reduction policy. Clémence Bourély | Nicolas Fortané | Didier Calavas | Agnès Leblond | Emilie Gay . Preventive Veterinary Medicine, 2018, 159, 23-134.

Highlights:

• Recourse to an antibiogram in veterinary medicine is multifactorial.
• Recourse to an antibiogram varies between animal sectors.
• Recourse to an antibiogram is influenced by relationship between stakeholders.
• The regulation of critically important antibiotics induces change in prescriptions.
• The regulation is perceived as an aid for promoting responsible use of antibiotics.

Highlights:

• The four major causative agents of canine otitis in France were coagulase-positive staphylococci, Pseudomonas aeruginosa, Proteus mirabilis and streptococci.
• Since 2013, resistance to fluoroquinolones has been on the decrease in both P. aeruginosa and Staphylococcus pseudintermedius isolates.
• For P. aeruginosa, 19.4% of isolates were resistant to both enrofloxacin and gentamicin.

Highlights:

• The five most frequently isolated bacteria were Streptococcus spp., Escherichia coli, Pseudomonas spp., Staphylococcus aureus, Pantoea spp. and Klebsiella spp.
• Klebsiella spp. and E. coli had the highest proportions of resistance to trimethoprim‐sulfamethoxazole
• Resistance to third‐generation cephalosporins was below 10% for all Enterobacteriaceae.
• The highest proportions of multidrug resistance (22.5%) were found among S. aureus isolates, which is worrying given their zoonotic potential.

Highlights:

• Resistances of P. multocida were almost all below 20% over the period 2012-2017.
• Resistance to florfenicol was less than 1%, regardless of the animal species.
• Resistance to fluoroquinolones increased in cattle isolates from 2012 to 2017.
• Similarly in cattle, other resistance trends continuously increased over the period.

Highlights:

• In total, 55,904 isolates were analyzed.
• Comparative analysis showed substantial agreement between MDR and co-resistance to amoxicillin and tetracycline with a kappa value of 0.75, all animal species considered.
• Given the widespread use of penicillins and tetracyclines in animal health, co-resistance to amoxicillin and tetracycline could be an efficient indicator of MDR in E. coli isolates.

OASIS evaluation of the French surveillance network for antimicrobial resistance in diseased animals (RESAPATH): success factors underpinning a well-performing voluntary system. Rodolphe Mader | Nathalie Jarrige | Marisa Haenni | Clémence Bourély | JY Madec | JP Amat. Epidemiol Infect. 2021 Apr 20;149:e104. doi: 10.1017/S0950268821000856.

Defining the scope of the European Antimicrobial Resistance Surveillance network in Veterinary medicine (EARS-Vet): a bottom-up and One Health approach. Rodolphe Mader | Clémence Bourély | JP Amat | Els M Broens | Luca Busani | et al. Journal of Antimicrobial Chemotherapy, 2022, dkab462, https://doi.org/10.1093/jac/dkab462.

French surveillance network for antimicrobial resistance in bacteria from diseased animals. Annual report.